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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
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- Product number
- 101-M24 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- VEGFR-1/Flt-1
- Antibody type
- Monoclonal
- Antigen
- Recombinant human soluble Flt-1(D1-5)
- Description
- antibody purified from hybridoma supernatant
- Reactivity
- Human, Mouse
- Host
- Mouse
- Antigen sequence
SKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKW
SLPEMVSKESERLSITKSACGRNGKQFCSTLTLNT
AQANHTGFYSCKYLAVPTSKKKETESAIYIFISDT
GRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNIT
VTLKKFPLDTLIPDGKRIIWDSRKGFIISNATYKE
IGLLTCEATVNGHLYKTNYLTHRQTNTIIDVQIST
PRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDE
KNKRASVRRRIDQSNSHANIFYSVLTIDKMQNKDK
GLYTCRVRSGPSFKSVNTSVHIYDKAFITVKHRKQ
QVLETVAGKRSYRLSMKVKAFPSPEVVWLKDGLPA
TEKSARYLTRGYSLIIKDVTEEDAGNYTILLSIKQ
SNVFKNLTATLIVNVKPQIYEKAVSSFPDPALYPL
GSRQILTCTAYGIPQPTIKWFWHPCNHNHSEARCD
FCSNNEESFILDADSNMGNRIESITQRMAIIEGKN
KMASTLVVADSRISGIYICIASNKVGTVGRNISFY
ITDVPNGFHVN- Isotype
- IgG
- Antibody clone number
- (#EWC)
- Vial size
- 100 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
Submitted references A unique and potent protein binding nature of liposome containing polyethylenimine and polyethylene Glycol: A nondisplaceable property
Vascular endothelial growth factor and its soluble receptor in benign and malignant ovarian tumors
Liu Y, Lin Y, Chen C, Lin C, Ma K, Chou F, Tsai J, Lin H, Chen F, Cheng T, Chang C, Liao K
Biotechnology and Bioengineering 2011 June;108(6):1318-1327
Biotechnology and Bioengineering 2011 June;108(6):1318-1327
Vascular endothelial growth factor and its soluble receptor in benign and malignant ovarian tumors
Artini P, Ruggiero M, Monteleone P, Carpi A, Cristello F, Cela V, Genazzani A
Biomedicine & Pharmacotherapy 2008 July;62(6):373-377
Biomedicine & Pharmacotherapy 2008 July;62(6):373-377
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western analysis of recombinant human and mouse soluble VEGF receptors using a monoclonal antibody directed against human recombinant sFlt-1(D5). There is a cross reactivity with mouse sFlt-1(D7)-Fc but no with human and mouse sKDR and human sFLT-4 visible.
- Sample type
- Purified recombinant proteins
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- The monoclonal anti-human VEGFR-1/Flt-1 antibody (#101-M24) shows a strong cross reactivity with recombinant mouse soluble VEGFR-1/Flt-1 in Western analysis.
- Sample type
- Sf9 conditioned media
