Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- BAF321 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal VEGFR1/Flt-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human VEGFR1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse VEGFR1, recombinant human (rh) VEGFR2, and rhVEGFR3 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Characteristics of the specific humoral response in patients with advanced solid tumors after active immunotherapy with a VEGF vaccine, at different antigen doses and using two distinct adjuvants.
Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease.
PEDF derived from glial Müller cells: a possible regulator of retinal angiogenesis.
Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
Sánchez Ramírez J, Morera Díaz Y, Bequet-Romero M, Hernández-Bernal F, Selman-Housein Bernal KH, de la Torre Santos A, Santiesteban Álvarez ER, Martín Bauta Y, Bermúdez Badell CH, de la Torre Pupo J, Gavilondo JV, CENTAURO-2 Team of Investigators, Ayala Avila M
BMC immunology 2017 Jul 26;18(1):39
BMC immunology 2017 Jul 26;18(1):39
Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease.
Bolnick JM, Kohan-Ghadr HR, Fritz R, Bolnick AD, Kilburn BA, Diamond MP, Armant DR, Drewlo S
Scientific reports 2016 Sep 23;6:32382
Scientific reports 2016 Sep 23;6:32382
PEDF derived from glial Müller cells: a possible regulator of retinal angiogenesis.
Eichler W, Yafai Y, Keller T, Wiedemann P, Reichenbach A
Experimental cell research 2004 Sep 10;299(1):68-78
Experimental cell research 2004 Sep 10;299(1):68-78
Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
European journal of clinical investigation 2003 Oct;33(10):883-90
European journal of clinical investigation 2003 Oct;33(10):883-90
Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
European journal of clinical investigation 2003 Oct;33(10):883-90
European journal of clinical investigation 2003 Oct;33(10):883-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- VEGF R1/Flt-1 in Human Breast. VEGF R1/Flt-1 was detected in immersion fixed paraffin-embedded sections of human breast array using Human VEGF R1/Flt-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF321) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.