PA5-95340

antibody from Invitrogen Antibodies

Targeting: SFRP4 FRP-4, frpHE, FRZB-2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-95340

Provider

Invitrogen Antibodies

Product name

SFRP4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Human SFRP4 shares 96.8% and 96.1% amino acid (aa) sequence identity with mouse and rat SFRP4, respectively.

Concentration

500 µg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SFRP4 in A549 whole cell lysates (lane 1) and SW620 whole cell lysates (lane 2). Samples were incubated with SFRP4 polyclonal antibody (Product # PA5-95340) using a 0.5 µg/mL dilution. developed was performed using enhanced chemiluminescence (ECL).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SFRP4 in, Lane 1: A549 whole cell lysates, Lane 2: SW620 whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1. 5 hour at RT. The membrane was incubated with SFRP4 Polyclonal Antibody (Product # PA5-95340) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for SFRP4 at approximately 49 kDa. The expected band size for SFRP4 is at 40 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SFRP4 in paraffin-embedded human endometrial carcinoma tissues. Samples were incubated with SFRP4 polyclonal antibody (Product # PA5-95340) at a 1 µg/mL dilution, and developed with Strepavidin-Biotin-Complex.