Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 711509 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DAP5 Recombinant Polyclonal Antibody (8HCLC)
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- This antibody is predicted to react with Bovine, Monkey, Cat
- Antibody clone number
- 8HCLC
- Concentration
- 0.5 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), A549 (Lane 2), SH-SY5Y (Lane 3), PC12 (Lane 4), A-431 (Lane 5), HL-60 (Lane 6), Jurkat (Lane 7), NIH/3T3 (Lane 8) and HeLa (Lane 9). The blots were probed with Anti-Dap5 Recombinant Rabbit Polyclonal Antibody (Product # 711509, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 102 kDa band corresponding to DAP5 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with wet transfer method. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).
