Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- TA319545 - Provider product page
- Provider
- OriGene
- Product name
- Mouse monoclonal MAD2L1 antibody
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal MAD2L1 antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MAD2L1
- Antibody clone number
- 17D10
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using anti-MAD2L1 antibody shows detection of a band at ~24 kDa (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate (lane 1). Approximately 75 ug of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4? with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5,000 dilution of IRDye?800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at RT.
- Validation comment
- WB