LF-MA0068

antibody from Invitrogen Antibodies

Targeting: PRDX1 NKEFA, PAGA

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

LF-MA0068

Provider

Invitrogen Antibodies

Product name

Anti-PRDX1 Monoclonal Antibody (2A4)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

A suggested positive control for this product is HeLa cells.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

2A4

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Fortilin potentiates the peroxidase activity of Peroxiredoxin-1 and protects against alcohol-induced liver damage in mice.

Chattopadhyay A, Pinkaew D, Doan HQ, Jacob RB, Verma SK, Friedman H, Peterson AC, Kuyumcu-Martinez MN, McDougal OM, Fujise K
Scientific reports 2016 Jan 4;6:18701

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Human skeletal muscle HSP70 response to training in highly trained rowers.

Liu Y, Mayr S, Opitz-Gress A, Zeller C, Lormes W, Baur S, Lehmann M, Steinacker JM
Journal of applied physiology (Bethesda, Md. : 1985) 1999 Jan;86(1):101-4

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Human skeletal muscle HSP70 response to training in highly trained rowers.

Liu Y, Mayr S, Opitz-Gress A, Zeller C, Lormes W, Baur S, Lehmann M, Steinacker JM
Journal of applied physiology (Bethesda, Md. : 1985) 1999 Jan;86(1):101-4

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Small subclass of rat olfactory neurons with specific bulbar projections is reactive with monoclonal antibodies to the HSP70 heat shock protein.

Carr VM, Murphy SP, Morimoto RI, Farbman AI
The Journal of comparative neurology 1994 Oct 1;348(1):150-60

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of PRDX1 was done on LNCap cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with PRDX1 Mouse Monoclonal Antibody (Product # LF-MA0068, red histogram) or with mouse isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.