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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
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- Product number
- LF-PA0004 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PRDX1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for this product is HeLa cells treated with H2O2.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Lot Dependent
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The Pentose Phosphate Pathway Regulates the Circadian Clock.
Thioredoxin-1/peroxiredoxin-1 as sensors of oxidative stress mediated by NADPH oxidase activity in atherosclerosis.
Peroxiredoxin-2 expression is increased in beta-thalassemic mouse red cells but is displaced from the membrane as a marker of oxidative stress.
Identification of cardiac myosin-binding protein C as a candidate biomarker of myocardial infarction by proteomics analysis.
Oxidation of 2-Cys-peroxiredoxins by arachidonic acid peroxide metabolites of lipoxygenases and cyclooxygenase-2.
Oxidation state governs structural transitions in peroxiredoxin II that correlate with cell cycle arrest and recovery.
Rey G, Valekunja UK, Feeney KA, Wulund L, Milev NB, Stangherlin A, Ansel-Bollepalli L, Velagapudi V, O'Neill JS, Reddy AB
Cell metabolism 2016 Sep 13;24(3):462-473
Cell metabolism 2016 Sep 13;24(3):462-473
Thioredoxin-1/peroxiredoxin-1 as sensors of oxidative stress mediated by NADPH oxidase activity in atherosclerosis.
Madrigal-Matute J, Fernandez-Garcia CE, Blanco-Colio LM, Burillo E, Fortuño A, Martinez-Pinna R, Llamas-Granda P, Beloqui O, Egido J, Zalba G, Martin-Ventura JL
Free radical biology & medicine 2015 Sep;86:352-61
Free radical biology & medicine 2015 Sep;86:352-61
Peroxiredoxin-2 expression is increased in beta-thalassemic mouse red cells but is displaced from the membrane as a marker of oxidative stress.
Matte A, Low PS, Turrini F, Bertoldi M, Campanella ME, Spano D, Pantaleo A, Siciliano A, De Franceschi L
Free radical biology & medicine 2010 Aug 1;49(3):457-66
Free radical biology & medicine 2010 Aug 1;49(3):457-66
Identification of cardiac myosin-binding protein C as a candidate biomarker of myocardial infarction by proteomics analysis.
Jacquet S, Yin X, Sicard P, Clark J, Kanaganayagam GS, Mayr M, Marber MS
Molecular & cellular proteomics : MCP 2009 Dec;8(12):2687-99
Molecular & cellular proteomics : MCP 2009 Dec;8(12):2687-99
Oxidation of 2-Cys-peroxiredoxins by arachidonic acid peroxide metabolites of lipoxygenases and cyclooxygenase-2.
Cordray P, Doyle K, Edes K, Moos PJ, Fitzpatrick FA
The Journal of biological chemistry 2007 Nov 9;282(45):32623-9
The Journal of biological chemistry 2007 Nov 9;282(45):32623-9
Oxidation state governs structural transitions in peroxiredoxin II that correlate with cell cycle arrest and recovery.
Phalen TJ, Weirather K, Deming PB, Anathy V, Howe AK, van der Vliet A, Jönsson TJ, Poole LB, Heintz NH
The Journal of cell biology 2006 Dec 4;175(5):779-89
The Journal of cell biology 2006 Dec 4;175(5):779-89
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PRDX1 Polyclonal Antibody (Product # LF-PA0004) and a 20KDa band corresponding to PRDX1 was observed upon treatment with Hydrogen peroxide. Whole cell extracts (30ug lysate) of HeLa (Lane 1), HeLa treated with Hydrogen peroxide (0.5mM, 2 hours) (Lane 2), MCF7 (Lane 3) and MCF7 treated with Hydrogen peroxide (0.5mM, 2 hours) (Lane 4) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
