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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-49912 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COL1A2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous levels of total COL1A2 protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COL1A2 in extracts from 293 cells and HeLa cells. Samples were probed using COL1A2 antibody (Product # PA5-49912).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COL1A2 in HeLa cells. Samples were probed using a COL1A2 polyclonal antibody (Product # PA5-49912).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of COL1A2 was performed on cryosections of human skin tissue. Tissues were blocked in 10% normal goat serum in 1X PBS containing 0.1% Triton X-100 (1X PBS-T) for 1 hour at room temperature (RT). The tissues were labeled with a COL1A2 polyclonal antibody (red, Product # PA5-49912) diluted 1:100 in 3% normal goat serum in 1X PBS-T for 1 hour at RT, followed by detection with a Goat anti-Rabbit IgG (H+L), Alexa Fluor 568 secondary antibody (Product # A-11036) diluted 1:2000 in 3% normal goat serum in 1X PBS-T for 1 hour at RT. Nuclei (blue) were stained with DAPI, included in ProLong Gold Anti-Fade Mountant (Product # P36931). Images were taken on an inverted microscope at 20X magnification. Data courtesy of Dr. Jiyoon Lee at Indiana University School of Medicine.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of COL1A2 in paraffin-embedded Human colon carcinoma tissue using COL1A2 Polyclonal Antibody (Product # PA5-49912).
