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LearnSTJ23259
antibody from St John's Laboratory
Targeting: CTCF
CFAP108, FAP108
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [6]
- Chromatin Immunoprecipitation [2]
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Validation data
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- Product number
- STJ23259 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-CTCF antibody (1-260) (STJ23259)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody anti-CTCF (1-260) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store in a freezer at-20°C and avoid freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using CTCF antibody (STJ23259) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of NIH/3T3 cells, using CTCF antibody (STJ23259) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using CTCF rabbit polyclonal antibody (STJ23259) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PC-12 cells using CTCF rabbit polyclonal antibody (STJ23259) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of U2OS cells using CTCF rabbit polyclonal antibody (STJ23259) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain using CTCF rabbit polyclonal antibody (STJ23259) at dilution of 1:100 (40x lens).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded Rat brain using CTCF rabbit polyclonal antibody (STJ23259) at dilution of 1:100 (40x lens).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded Human colon using CTCF rabbit polyclonal antibody (STJ23259) at dilution of 1:100 (40x lens).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug CTCF antibody (STJ23259). Western blot was performed from the immunoprecipitate using CTCF antibody (STJ23259) at a dilition of 1:500.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis of extracts of HeLa cells, using CTCF antibody (STJ23259) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
