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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- GTX48814 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48814, RRID:AB_11171564
- Product name
- IL21 antibody
- Antibody type
- Polyclonal
- Reactivity
- Bovine, Horse, Porcine
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's anti-bovine IL-21 antibody shows detection of recombinant bovine IL-21 at 15.1kDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with GeneTex's, Inc. Anti-bovine IL-21. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's anti-bovine IL-21 antibody shows detection of recombinant bovine IL-21 at 15.1kDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA , and probed with GeneTex's anti-bovine IL-21 antibody. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.
