NBP2-16589

antibody from Novus Biologicals

Targeting: LGALS3 GALIG, LGALS2, MAC-2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-16589

Provider

Novus Biologicals

Product name

Rabbit Polyclonal Galectin-3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Galectin-3 Antibody [NBP2-16589] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: LGALS3 transfected 293T lysates 12% SDS PAGE gel, diluted at 1:10000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Galectin-3 Antibody [NBP2-16589] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE gel, diluted at 1:10000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Galectin-3 Antibody [NBP2-16589] - Wild-type (WT) and Galectin 3 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Galectin-3 Antibody [NBP2-16589] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Galectin-3 Antibody [NBP2-16589] - Galectin-3 antibody detects Galectin-3 protein by western blot analysis. A. 50 ug rat colon lysate/extract. 12% SDS-PAGE. Galectin-3 antibody dilution: 1:10000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Galectin-3 Antibody [NBP2-16589] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Sandwich ELISA: Galectin-3 Antibody [NBP2-16589] - Sandwich ELISA detection of recombinant full-length Galectin-3 protein using as capture antibody at concentration of 5 ug/mL and Galectin-3 Antibody as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP2-16589] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.