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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 677301 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#677301, RRID:AB_2565901
- Product name
- Purified anti-Mac-2 (Galectin-3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length human Galectin-3.
- Description
- Galectin-3 (galactose-specific lectin 3) is a unique member of the β-galactoside-binding lectin family molecule, which plays an important role in regulation of cell proliferation, differentiation, and tumor cell metastasis. Galectin-3 is mainly found in the cytoplasm, but it is also seen in the nucleus and can be secreted by the non-classical, secretory pathway. In general, secreted galectin-3 mediates cell migration, cell adhesion, and cell-cell interactions through its high affinity binding with galactose-containing glycoproteins on the cell surface. Cytoplasmic galectin-3 exhibits anti-apoptotic activity and regulates several signal transduction pathways, whereas nuclear galectin-3 has been associated with pre-mRNA splicing and gene expression. Recent studies also indicate the potential role of galectin-3 as a biomarker for acute and chronic heart failure.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- M1606B04
- Vial size
- 50 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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- Experimental details
- Western blot analysis of MCF-7 (lane 1), HeLa (lane 2), and 3T3-L1 (lane 3) cell lysates. The blots were probed with anti-Mac-2 (Galectin-3) antibody (clone M1606B04) and visualized using HRP anti-rat IgG antibody. β-actin antibody (clone Poly6221) was used as a loading control.
- Conjugate
- Unconjugated
