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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- BAF1197 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Galectin-3 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Galectin-3 in ELISAs and Western blots. In sandwich ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Galectin-3 is observed and less than 0.05% cross-reactivity with recombinant mouse (rm) Galectin-1, rhGalectin-2, and rhGalectin-4 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
NP_034835- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Galectin-3 in Mouse Cartilage. Galectin-3 was detected in immersion fixed frozen sections of mouse cartilage using 15 µg/mL Goat Anti-Mouse Galectin-3 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1197) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
