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- Antigen structure
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- Western blot [1]
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- Product number
- MAB1154 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Galectin-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Galectin-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows 100% cross-reactivity with recombinant mouse Galectin-3 and no cross-reactivity with rhGalectin-1, -2 -4, -7, or -8.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P17931- Isotype
- IgG
- Antibody clone number
- 194804
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A role for collagen XXIII in cancer cell adhesion, anchorage-independence and metastasis.
Galectin-1-mediated cell death is increased by CD30-induced signaling in anaplastic large cell lymphoma cells but not in Hodgkin lymphoma cells.
Galectin-3 is an amplifier of inflammation in atherosclerotic plaque progression through macrophage activation and monocyte chemoattraction.
Spivey KA, Chung I, Banyard J, Adini I, Feldman HA, Zetter BR
Oncogene 2012 May 3;31(18):2362-72
Oncogene 2012 May 3;31(18):2362-72
Galectin-1-mediated cell death is increased by CD30-induced signaling in anaplastic large cell lymphoma cells but not in Hodgkin lymphoma cells.
Suzuki O, Hirsch B, Abe M, Dürkop H, Stein H
Laboratory investigation; a journal of technical methods and pathology 2012 Feb;92(2):191-9
Laboratory investigation; a journal of technical methods and pathology 2012 Feb;92(2):191-9
Galectin-3 is an amplifier of inflammation in atherosclerotic plaque progression through macrophage activation and monocyte chemoattraction.
Papaspyridonos M, McNeill E, de Bono JP, Smith A, Burnand KG, Channon KM, Greaves DR
Arteriosclerosis, thrombosis, and vascular biology 2008 Mar;28(3):433-40
Arteriosclerosis, thrombosis, and vascular biology 2008 Mar;28(3):433-40
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Galectin-3 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Galectin-3 Monoclonal Antibody (Catalog # MAB1154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
