PA5-30028

antibody from Invitrogen Antibodies

Targeting: FBP1 FBP

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30028

Provider

Invitrogen Antibodies

Product name

FBP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: THP-1, HL-60.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FBP1 using 30 µg of A) THP-1 and B) HL-60 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a FBP1 polyclonal antibody (Product # PA5-30028) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), Hep G2 (Lane 2), HCT 116 (Lane 3), SW-480 (Lane 4), tissue extracts of Mouse Liver (Lane 5), Mouse Colon (Lane 6) and Mouse Skeletal Muscle (Lane 7). The blot was probed with Anti-FBP1 Polyclonal Antibody (Product # PA5-30028, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 ug/ml, 1:4000 dilution). A 39 kDa band corresponding to FBP1 was observed across cell line and tissues tested except in Hep G2, HCT 116 and SW-480 (low levels due to promoter methylation). Additional non specific bands were seen around 50kDa in Mouse Liver and Mouse Colon.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using FBP1 Polyclonal Antibody (Product # PA5-30028). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with FBP1 Polyclonal Antibody (Product # PA5-30028) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.