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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- AF1878 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal SHP-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat SHP-1. The antibody does not detect SHP-2 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references S100A8 induces IL-10 and protects against acute lung injury.
Hiroshima Y, Hsu K, Tedla N, Chung YM, Chow S, Herbert C, Geczy CL
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 15;192(6):2800-11
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 15;192(6):2800-11
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat SHP-1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat SHP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1878) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active SHP-1 aa 205-595 (Catalog # 1878-SH) and Recombinant Human SHP-2 (Catalog # 1894-SH) (2 ng/lane) were included. A specific band for SHP-1 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human SHP-1 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SHP-1 at approximately 65 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat SHP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1878) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
