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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA302110 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA302110, RRID:AB_2161349
- Product name
- Rabbit Polyclonal Antibody against PDK2 (C-term)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Antibody against PDK2 (C-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PDK2
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of PDK2 Antibody (C-term)(Cat. #TA302110) in mouse intestine tissue lysate (35ug/lane). PDK2 (arrow) was detected using the purified Pab.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of PDK2 (arrow) using PDK2 Antibody (C-term)(Cat. #TA302110). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK2 gene (Lane 2) (Origene Technologies).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- PDK2 Antibody (C392) (Cat. #TA302110) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PDK2 antibody detected the PDK2 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
- Validation comment
- IHC
