MA5-23524

antibody from Invitrogen Antibodies

Targeting: ME1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23524

Provider

Invitrogen Antibodies

Product name

ME1 Monoclonal Antibody (3I6)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: 293T, A431, and HeLa cells.

Antibody clone number

3I6

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ME1 in various whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with a ME1 monoclonal antibody (Product # MA5-23524) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ME1 Polyclonal Antibody detects ME1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 Polyclonal Antibody ME1 Monoclonal Antibody (3I6) (Product # MA5-23524) diluted at a dilution of 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ME1 Polyclonal Antibody detects ME1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 Polyclonal Antibody ME1 Monoclonal Antibody (3I6) (Product # MA5-23524) diluted at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Double-labeled immunohistochemistry photomicrographs of frozen sections of mouse brain. Green: Vimentin antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Red: ME1 Monoclonal Antibody (3I6) (Product # MA5-23524) diluted at 1:200. The signal was developed using goat anti-mouse IgG antibody (Dylight594). Blue: Nuclear staining with Hoechst 33342. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ME1 Monoclonal Antibody (3I6) detects ME1 protein at cytoplasm in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. ME1 Monoclonal Antibody (3I6) (Product # MA5-23524) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ME1 Monoclonal Antibody (3I6) detects ME1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ME1 Monoclonal Antibody (3I6) (Product # MA5-23524) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.