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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Product number
- ABIN358103 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6) (pSer54) antibody
- Antibody type
- Polyclonal
- Description
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- pSer54
- Vial size
- 0.4 mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Cdc6 chromatin affinity is unaffected by serine-54 phosphorylation, S-phase progression, and overexpression of cyclin A.
Caspase-3-mediated cleavage of Cdc6 induces nuclear localization of p49-truncated Cdc6 and apoptosis.
Human replication protein Cdc6 prevents mitosis through a checkpoint mechanism that implicates Chk1.
Regulation of CDC6, geminin, and CDT1 in human cells that undergo polyploidization.
Human replication protein Cdc6 is selectively cleaved by caspase 3 during apoptosis.
Alexandrow MG, Hamlin JL
Molecular and cellular biology 2004 Feb;24(4):1614-27
Molecular and cellular biology 2004 Feb;24(4):1614-27
Caspase-3-mediated cleavage of Cdc6 induces nuclear localization of p49-truncated Cdc6 and apoptosis.
Yim H, Jin YH, Park BD, Choi HJ, Lee SK
Molecular biology of the cell 2003 Oct;14(10):4250-9
Molecular biology of the cell 2003 Oct;14(10):4250-9
Human replication protein Cdc6 prevents mitosis through a checkpoint mechanism that implicates Chk1.
Clay-Farrace L, Pelizon C, Santamaria D, Pines J, Laskey RA
The EMBO journal 2003 Feb 3;22(3):704-12
The EMBO journal 2003 Feb 3;22(3):704-12
Regulation of CDC6, geminin, and CDT1 in human cells that undergo polyploidization.
Bermejo R, Vilaboa N, Calés C
Molecular biology of the cell 2002 Nov;13(11):3989-4000
Molecular biology of the cell 2002 Nov;13(11):3989-4000
Human replication protein Cdc6 is selectively cleaved by caspase 3 during apoptosis.
Pelizon C, d'Adda di Fagagna F, Farrace L, Laskey RA
EMBO reports 2002 Aug;3(8):780-4
EMBO reports 2002 Aug;3(8):780-4
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