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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AM2067a - Provider product page
- Provider
- Abgent
- Product name
- FASN Antibody (Center) (Ascites)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgM
- Vial size
- 100 µl
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- FASN Antibody (Center) (Cat. #AM2067a) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the FASN antibody detected the FASN protein (arrow).
- Primary Ab dilution
- 1:100~1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Fluorescent confocal image of HepG2 cells stained with FASN (Center) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM2067a FASN primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ¼g/ml, 5 min). Note the highly specific localization of the FASN immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000169710)."
- Primary Ab dilution
- 1:200
