MA5-27013

antibody from Invitrogen Antibodies

Targeting: ZNF449 FLJ23614, ZSCAN19

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27013

Provider

Invitrogen Antibodies

Product name

ZNF449 Monoclonal Antibody (OTI4C6)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI4C6

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ZNF449 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with ZNF449 (Product # MA5-27013) monoclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ZNF449 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with ZNF449 (Product # MA5-27013) monoclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded carcinoma of human kidney tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a ZNF449 monoclonal antibody (Product # MA5-27013) at a dilution of 1:150.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded carcinoma of human lung tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a ZNF449 monoclonal antibody (Product # MA5-27013) at a dilution of 1:150.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human bladder tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA, pH8.0. Following antigen retrieval, tissues were probed with a ZNF449 monoclonal antibody (Product # MA5-27013) at a dilution of 1:150.