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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- OAPC00021 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- Anti-Nestin
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4D11
- Vial size
- 100µl
- Storage
- Store at -20°C in undiluted aliquots; stable for up to one year after date of receipt. Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 k nestin doublet.
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Immunochemical staining of cultured neonatal rat neurons and glia showing nestin labeling in red and vimentin (cat. # OAPC00084) in green. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
