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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- ABIN1580439 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Nestin, Intermediate Filament, Marker of Neuronal Stem Cells antibody
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- ascites fluid
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4D11
- Vial size
- 100 μL
- Storage
- Store at 4°C short term or -20°C long term.
- Handling
- Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot in of developing rat brain (P18) homogenate probe with ABIN1580439. A single strong band running at ~240 kDa is seen.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Mixed cultures of neonatal rat neurons and glia stained with ABIN1580439 (red), chicken antibody to vimentin CPCA-Vim (green) and DNA (DAPI stain, blue). Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the ABIN1580439 antibody. The filamentous staining pattern is as expected as both Nestin and vimentin are components of 10nm filaments. Note that some cells contain Nestin, but do not stain strongly for vimentin and so appear red. Others contain vimentin and not Nestin and so appear green- these are likely to be fibroblastic or endothelial cells. Some cells express both proteins and so appear yellowish. The presence of nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.
