Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 656804 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#656804, RRID:AB_2563509
- Product name
- Alexa Fluor® 594 anti-Nestin
- Antibody type
- Monoclonal
- Antigen
- Fusion protein
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- 10C2
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
No comments: Submit comment
Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- HUVEC cells were fixed with 1% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100, and blocked with 5% fetal bovine serum for 30 minutes at room temperature. Then the cells were stained with 5 µg/ml of anti-human Nestin (clone 10C2) Alexa Fluor® 594 overnight followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
- Conjugate
- Yellow dye
