MA5-23650

antibody from Invitrogen Antibodies

Targeting: NES FLJ21841

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23650

Provider

Invitrogen Antibodies

Product name

Nestin Monoclonal Antibody (196908), APC

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

196908

Vial size

100 Tests

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

Combining HDAC and MEK Inhibitors with Radiation against Glioblastoma-Derived Spheres.

Essien EI, Hofer TP, Atkinson MJ, Anastasov N
Cells 2022 Feb 23;11(5)

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Generation of two H1 hESC sublines carrying deletions of RB1 exon 1/promoter in heterozygous or compound heterozygous state.

Menges J, Cremanns M, Steenpass L
Stem cell research 2019 Aug;39:101517

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Generation of heterozygous and homozygous hESC H9 sublines carrying inactivating mutations in RB1.

Schipper L, Kanber D, Steenpass L
Stem cell research 2018 Dec;33:41-45

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Nestin in NTera‚2 human testicular embryonic carcinoma cell line. Samples were incubated in Nestin monoclonal antibody (Product # MA5-23650) or isotype control.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Single expression of GSLC markers reduced by the combination of HDAC and MEK inhibitor with radiation. ( a ) Representative example of flow cytometric histograms of Nestin in U251-sph 72 h after the spheres were treated with HDCAi (1 uM MS-275), MEKi (1 uM TAK-733 or 1 uM trametinib), a combination of both (1 uM MS-275 + 1 uM TAK-733 or 1 uM MS-275 + 1 uM trametinib) and 50 uM TMZ alone or with 4 Gy radiation. Upper histograms represent the unstained sample and fluorescence minus one (FMO) control for the gating percentage of Nestin-positive cells (Nestin+). Lower histograms show the percentage of Nestin-positive cells after the indicated treatment conditions and 4 Gy irradiation. Values inside each histogram represent the percentage of positive single cells from a total of approximately 2 x 10 4 cells acquired. ( b ) Quantification of CD44+, Nestin+ and SOX2+ relative to sham irradiated control cells (DMSO) set to 100% in U251-sph and ( c ) U87-sph. Data represent means +- SEM ( n = 3). Asterisks indicate significant differences between 0 Gy DMSO and treated samples using Student's t -test: * p