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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NBP2-59775 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Nestin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Specificity of human Nestin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
VGGLGDPGHL- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nestin Antibody (CL0197) [NBP2-59775] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251 MG
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nestin Antibody (CL0197) [NBP2-59775] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-NES monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775] - Staining of human cerebral cortex shows strong immunoreactivity in the endothelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775] - Staining of human kidney shows strong positivity in renal glomeruli.
