Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF1339 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Galectin-7 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Galectin-7 in ELISAs and Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant mouse (rm) Galectin-7 is observed and less than 0.5% cross-reactivity with recombinant human (rh) Galectin-1, rmGalectin-3, rhGalectin-4, and rhGalectin-8 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Tissue and plasma levels of galectins in patients with high grade serous ovarian carcinoma as new predictive biomarkers.
A Mutation in the Carbohydrate Recognition Domain Drives a Phenotypic Switch in the Role of Galectin-7 in Prostate Cancer.
The CCAAT/enhancer-binding protein beta-2 isoform (CEBPβ-2) upregulates galectin-7 expression in human breast cancer cells.
Galectin-7 acts as an adhesion molecule during implantation and increased expression is associated with miscarriage.
Labrie M, De Araujo LOF, Communal L, Mes-Masson AM, St-Pierre Y
Scientific reports 2017 Oct 16;7(1):13244
Scientific reports 2017 Oct 16;7(1):13244
A Mutation in the Carbohydrate Recognition Domain Drives a Phenotypic Switch in the Role of Galectin-7 in Prostate Cancer.
Labrie M, Vladoiu M, Leclerc BG, Grosset AA, Gaboury L, Stagg J, St-Pierre Y
PloS one 2015;10(7):e0131307
PloS one 2015;10(7):e0131307
The CCAAT/enhancer-binding protein beta-2 isoform (CEBPβ-2) upregulates galectin-7 expression in human breast cancer cells.
Campion CG, Labrie M, Grosset AA, St-Pierre Y
PloS one 2014;9(5):e95087
PloS one 2014;9(5):e95087
Galectin-7 acts as an adhesion molecule during implantation and increased expression is associated with miscarriage.
Menkhorst EM, Gamage T, Cuman C, Kaitu'u-Lino TJ, Tong S, Dimitriadis E
Placenta 2014 Mar;35(3):195-201
Placenta 2014 Mar;35(3):195-201
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Galectin-7 by Simple Western<sup abp="265">TM. Simple Western lane view shows lysates of HEK001 human epidermal keratinocyte cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-7 at approximately 21 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1339) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Galectin-7 by Western Blot. Western blot shows lysates of human skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1339) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin-7 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.