22-0306-71

antibody from Invitrogen Antibodies

Targeting: CD8B CD8B1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

22-0306-71

Provider

Invitrogen Antibodies

Product name

CD3/CD4/CD8a Antibody Cocktail, APC, FITC, PerCP-eFluor™ 710, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This three-color cocktail is designed to identify T cells from peripheral blood samples. It can be used alone, or in combination with other antibodies to further characterize T cell populations. The SK3 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The SK7 monoclonal antibody reacts with human CD3 eplison, a 20 kDa subunit of the CD3/TCR complex. The CD3 complex, including the epsilon, gamma and delta subunits, is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK1 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8beta chain (CD8alpha beta) or as a homodimer (CD8alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a.

Antibody clone number

SK3, SK7, SK1

Concentration

20 µL/Test

References

Submitted references

Immunological factors, but not clinical features, predict visceral leishmaniasis relapse in patients co-infected with HIV.

Takele Y, Mulaw T, Adem E, Shaw CJ, Franssen SU, Womersley R, Kaforou M, Taylor GP, Levin M, Müller I, Cotton JA, Kropf P
Cell reports. Medicine 2022 Jan 18;3(1):100487

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Differences in Maturation Status and Immune Phenotypes of Circulating Helios(+) and Helios(-) Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Zhang Y, Zhang J, Shi Y, Shen M, Lv H, Chen S, Feng Y, Chen H, Xu X, Yang T, Xu K
Frontiers in immunology 2021;12:628504

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Mucosal integrin α4β7 blockade fails to reduce the seeding and size of viral reservoirs in SIV-infected rhesus macaques.

Ziani W, Shao J, Fang A, Connolly PJ, Wang X, Veazey RS, Xu H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Feb;35(2):e21282

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human peripheral blood cells with eZfluor Anti-Human CD4 FITC, CD3 PerCP-eFluor® 710, CD8a APC. Red blood cells were lysed using the 10X RBC Lysis Buffer (Multi-Species) (Product # 00-4300-54). Cells in the lymphocyte gate were used for analysis.