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LearnIC7821P-100
antibody from R&D Systems
Targeting: IL22
IL-21, IL-22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFa, TIFIL-23, zcyto18
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [29]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- IC7821P-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-22 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-22 in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse IL-22 and recombinant rat IL-22 and no cross-reactivity with recombinant human IL-10.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
Q9GZX6- Isotype
- IgG
- Antibody clone number
- 142928
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Mass cytometry reveals innate lymphoid cell differentiation pathways in the human fetal intestine.
A20 upregulation during treated HIV disease is associated with intestinal epithelial cell recovery and function.
Genetic deficiency of NOD2 confers resistance to invasive aspergillosis.
RNA-seq and flow-cytometry of conventional, scalp, and palmoplantar psoriasis reveal shared and distinct molecular pathways.
Enhancing adoptive cancer immunotherapy with Vγ2Vδ2 T cells through pulse zoledronate stimulation.
Skin-infiltrating, interleukin-22-producing T cells differentiate pediatric psoriasis from adult psoriasis.
High frequency of intestinal TH17 cells correlates with microbiota alterations and disease activity in multiple sclerosis.
IL-22-producing CD4+T cells in the treatment response of rheumatoid arthritis to combination therapy with methotrexate and leflunomide.
Composition and functionality of the intrahepatic innate lymphoid cell-compartment in human nonfibrotic and fibrotic livers.
Expansion of Th17 Cells by Human Mast Cells Is Driven by Inflammasome-Independent IL-1β.
Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vγ2Vδ2 T cells after Mycobacterium tuberculosis infection or vaccination.
The effect of aging on the frequency, phenotype and cytokine production of human blood CD4 + CXCR5 + T follicular helper cells: comparison of aged and young subjects.
Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients.
CX₃CR1⁺ mononuclear phagocytes support colitis-associated innate lymphoid cell production of IL-22.
A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis.
IL-26 is overexpressed in rheumatoid arthritis and induces proinflammatory cytokine production and Th17 cell generation.
Functionally distinct subsets of human FOXP3+ Treg cells that phenotypically mirror effector Th cells.
ESAT-6- and CFP-10-specific Th1, Th22 and Th17 cells in tuberculous pleurisy may contribute to the local immune response against Mycobacterium tuberculosis infection.
Interleukin-21 induces the differentiation of human Tc22 cells via phosphorylation of signal transducers and activators of transcription.
Activation of the aryl hydrocarbon receptor reveals distinct requirements for IL-22 and IL-17 production by human T helper cells.
Overrepresentation of IL-17A and IL-22 producing CD8 T cells in lesional skin suggests their involvement in the pathogenesis of psoriasis.
CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses.
Chronic mucocutaneous candidiasis in APECED or thymoma patients correlates with autoimmunity to Th17-associated cytokines.
IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells.
Stage 3 immature human natural killer cells found in secondary lymphoid tissue constitutively and selectively express the TH 17 cytokine interleukin-22.
Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers.
Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
Li N, van Unen V, Höllt T, Thompson A, van Bergen J, Pezzotti N, Eisemann E, Vilanova A, Chuva de Sousa Lopes SM, Lelieveldt BPF, Koning F
The Journal of experimental medicine 2018 May 7;215(5):1383-1396
The Journal of experimental medicine 2018 May 7;215(5):1383-1396
A20 upregulation during treated HIV disease is associated with intestinal epithelial cell recovery and function.
Chitre AS, Kattah MG, Rosli YY, Pao M, Deswal M, Deeks SG, Hunt PW, Abdel-Mohsen M, Montaner LJ, Kim CC, Ma A, Somsouk M, McCune JM
PLoS pathogens 2018 Mar;14(3):e1006806
PLoS pathogens 2018 Mar;14(3):e1006806
Genetic deficiency of NOD2 confers resistance to invasive aspergillosis.
Gresnigt MS, Cunha C, Jaeger M, Gonçalves SM, Malireddi RKS, Ammerdorffer A, Lubbers R, Oosting M, Rasid O, Jouvion G, Fitting C, Jong DJ, Lacerda JF, Campos A Jr, Melchers WJG, Lagrou K, Maertens J, Kanneganti TD, Carvalho A, Ibrahim-Granet O, van de Veerdonk FL
Nature communications 2018 Jul 6;9(1):2636
Nature communications 2018 Jul 6;9(1):2636
RNA-seq and flow-cytometry of conventional, scalp, and palmoplantar psoriasis reveal shared and distinct molecular pathways.
Ahn R, Yan D, Chang HW, Lee K, Bhattarai S, Huang ZM, Nakamura M, Singh R, Afifi L, Taravati K, Munoz-Sandoval P, Pauli M, Rosenblum MD, Liao W
Scientific reports 2018 Jul 27;8(1):11368
Scientific reports 2018 Jul 27;8(1):11368
Enhancing adoptive cancer immunotherapy with Vγ2Vδ2 T cells through pulse zoledronate stimulation.
Nada MH, Wang H, Workalemahu G, Tanaka Y, Morita CT
Journal for immunotherapy of cancer 2017;5:9
Journal for immunotherapy of cancer 2017;5:9
Skin-infiltrating, interleukin-22-producing T cells differentiate pediatric psoriasis from adult psoriasis.
Cordoro KM, Hitraya-Low M, Taravati K, Sandoval PM, Kim E, Sugarman J, Pauli ML, Liao W, Rosenblum MD
Journal of the American Academy of Dermatology 2017 Sep;77(3):417-424
Journal of the American Academy of Dermatology 2017 Sep;77(3):417-424
High frequency of intestinal TH17 cells correlates with microbiota alterations and disease activity in multiple sclerosis.
Cosorich I, Dalla-Costa G, Sorini C, Ferrarese R, Messina MJ, Dolpady J, Radice E, Mariani A, Testoni PA, Canducci F, Comi G, Martinelli V, Falcone M
Science advances 2017 Jul;3(7):e1700492
Science advances 2017 Jul;3(7):e1700492
IL-22-producing CD4+T cells in the treatment response of rheumatoid arthritis to combination therapy with methotrexate and leflunomide.
Zhong W, Zhao L, Liu T, Jiang Z
Scientific reports 2017 Jan 24;7:41143
Scientific reports 2017 Jan 24;7:41143
Composition and functionality of the intrahepatic innate lymphoid cell-compartment in human nonfibrotic and fibrotic livers.
Forkel M, Berglin L, Kekäläinen E, Carlsson A, Svedin E, Michaëlsson J, Nagasawa M, Erjefält JS, Mori M, Flodström-Tullberg M, Bergquist A, Ljunggren HG, Westgren M, Lindforss U, Friberg D, Jorns C, Ellis E, Björkström NK, Mjösberg J
European journal of immunology 2017 Aug;47(8):1280-1294
European journal of immunology 2017 Aug;47(8):1280-1294
Expansion of Th17 Cells by Human Mast Cells Is Driven by Inflammasome-Independent IL-1β.
Suurmond J, Habets KL, Dorjée AL, Huizinga TW, Toes RE
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4473-4481
Journal of immunology (Baltimore, Md. : 1950) 2016 Dec 1;197(11):4473-4481
Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova JL, Puel A
The Journal of experimental medicine 2015 May 4;212(5):619-31
The Journal of experimental medicine 2015 May 4;212(5):619-31
Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vγ2Vδ2 T cells after Mycobacterium tuberculosis infection or vaccination.
Shen H, Wang Y, Chen CY, Frencher J, Huang D, Yang E, Ryan-Payseur B, Chen ZW
European journal of immunology 2015 Feb;45(2):442-51
European journal of immunology 2015 Feb;45(2):442-51
The effect of aging on the frequency, phenotype and cytokine production of human blood CD4 + CXCR5 + T follicular helper cells: comparison of aged and young subjects.
Zhou M, Zou R, Gan H, Liang Z, Li F, Lin T, Luo Y, Cai X, He F, Shen E
Immunity & ageing : I & A 2014;11:12
Immunity & ageing : I & A 2014;11:12
Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients.
Teunissen MBM, Munneke JM, Bernink JH, Spuls PI, Res PCM, Te Velde A, Cheuk S, Brouwer MWD, Menting SP, Eidsmo L, Spits H, Hazenberg MD, Mjösberg J
The Journal of investigative dermatology 2014 Sep;134(9):2351-2360
The Journal of investigative dermatology 2014 Sep;134(9):2351-2360
CX₃CR1⁺ mononuclear phagocytes support colitis-associated innate lymphoid cell production of IL-22.
Longman RS, Diehl GE, Victorio DA, Huh JR, Galan C, Miraldi ER, Swaminath A, Bonneau R, Scherl EJ, Littman DR
The Journal of experimental medicine 2014 Jul 28;211(8):1571-83
The Journal of experimental medicine 2014 Jul 28;211(8):1571-83
A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis.
Gironi M, Saresella M, Rovaris M, Vaghi M, Nemni R, Clerici M, Grossi E
Immunity & ageing : I & A 2013 Jan 10;10(1):1
Immunity & ageing : I & A 2013 Jan 10;10(1):1
IL-26 is overexpressed in rheumatoid arthritis and induces proinflammatory cytokine production and Th17 cell generation.
Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barré B, Audran M, Bouvard B, Saint-André JP, Jeannin P
PLoS biology 2012;10(9):e1001395
PLoS biology 2012;10(9):e1001395
Functionally distinct subsets of human FOXP3+ Treg cells that phenotypically mirror effector Th cells.
Duhen T, Duhen R, Lanzavecchia A, Sallusto F, Campbell DJ
Blood 2012 May 10;119(19):4430-40
Blood 2012 May 10;119(19):4430-40
ESAT-6- and CFP-10-specific Th1, Th22 and Th17 cells in tuberculous pleurisy may contribute to the local immune response against Mycobacterium tuberculosis infection.
Qiao D, Yang BY, Li L, Ma JJ, Zhang XL, Lao SH, Wu CY
Scandinavian journal of immunology 2011 Apr;73(4):330-7
Scandinavian journal of immunology 2011 Apr;73(4):330-7
Interleukin-21 induces the differentiation of human Tc22 cells via phosphorylation of signal transducers and activators of transcription.
Liu Y, Yang B, Ma J, Wang H, Huang F, Zhang J, Chen H, Wu C
Immunology 2011 Apr;132(4):540-8
Immunology 2011 Apr;132(4):540-8
Activation of the aryl hydrocarbon receptor reveals distinct requirements for IL-22 and IL-17 production by human T helper cells.
Ramirez JM, Brembilla NC, Sorg O, Chicheportiche R, Matthes T, Dayer JM, Saurat JH, Roosnek E, Chizzolini C
European journal of immunology 2010 Sep;40(9):2450-9
European journal of immunology 2010 Sep;40(9):2450-9
Overrepresentation of IL-17A and IL-22 producing CD8 T cells in lesional skin suggests their involvement in the pathogenesis of psoriasis.
Res PC, Piskin G, de Boer OJ, van der Loos CM, Teeling P, Bos JD, Teunissen MB
PloS one 2010 Nov 24;5(11):e14108
PloS one 2010 Nov 24;5(11):e14108
CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses.
Carbone T, Nasorri F, Pennino D, Eyerich K, Foerster S, Cifaldi L, Traidl-Hoffman C, Behrendt H, Cavani A
Journal of immunology (Baltimore, Md. : 1950) 2010 Jan 15;184(2):1102-10
Journal of immunology (Baltimore, Md. : 1950) 2010 Jan 15;184(2):1102-10
Chronic mucocutaneous candidiasis in APECED or thymoma patients correlates with autoimmunity to Th17-associated cytokines.
Kisand K, Bøe Wolff AS, Podkrajsek KT, Tserel L, Link M, Kisand KV, Ersvaer E, Perheentupa J, Erichsen MM, Bratanic N, Meloni A, Cetani F, Perniola R, Ergun-Longmire B, Maclaren N, Krohn KJ, Pura M, Schalke B, Ströbel P, Leite MI, Battelino T, Husebye ES, Peterson P, Willcox N, Meager A
The Journal of experimental medicine 2010 Feb 15;207(2):299-308
The Journal of experimental medicine 2010 Feb 15;207(2):299-308
IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
The Journal of allergy and clinical immunology 2009 Jan;123(1):59-66.e4
The Journal of allergy and clinical immunology 2009 Jan;123(1):59-66.e4
Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells.
Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F
Nature immunology 2009 Aug;10(8):857-63
Nature immunology 2009 Aug;10(8):857-63
Stage 3 immature human natural killer cells found in secondary lymphoid tissue constitutively and selectively express the TH 17 cytokine interleukin-22.
Hughes T, Becknell B, McClory S, Briercheck E, Freud AG, Zhang X, Mao H, Nuovo G, Yu J, Caligiuri MA
Blood 2009 Apr 23;113(17):4008-10
Blood 2009 Apr 23;113(17):4008-10
Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers.
Nistala K, Moncrieffe H, Newton KR, Varsani H, Hunter P, Wedderburn LR
Arthritis and rheumatism 2008 Mar;58(3):875-87
Arthritis and rheumatism 2008 Mar;58(3):875-87
Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
Nature immunology 2007 Sep;8(9):942-9
Nature immunology 2007 Sep;8(9):942-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-22 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Ca++ Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human IL-22 PE-conjugated Monoclonal Antibody (Catalog # IC7821P) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
