46-7229-41
antibody from Invitrogen Antibodies
Targeting: IL22
IL-21, IL-22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFa, TIFIL-23, zcyto18
Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 46-7229-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-IL-22 Monoclonal Antibody (22URTI), PerCP-eFluor 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 22URTI monoclonal antibody reacts with human interleukin(IL)-22. IL-22 is a 20 kDa member of the IL-10 cytokine family that is secreted primarily by Th17 and NK cells. Nevertheless, other T cells have also been shown to produce IL-22. In in vitro Th17 cultures, induction of IL-22 expression is greater in response to IL-23 than IL-6 or TGF beta, suggesting that this cytokine may be secreted by more fully differentiated Th17 cells in vivo. A heterodimer consisting of IL-10R2 and IL-22R1 serves as the receptor for IL-22. Applications Reported: This 22URTI antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 22URTI antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of restimulated, Th17-polarized CD4+ human peripheral blood mononuclear cells (normal human peripheral blood cells) or restimulated total normal human peripheral blood cells. This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 22URTI
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Broadly reactive human CD4+ T cells against Enterobacteriaceae are found in the naïve repertoire and are clonally expanded in the memory repertoire.
An immunoregulatory and tissue-residency program modulated by c-MAF in human TH17 cells.
Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.
Th1 is the predominant helper T cell subset that produces GM-CSF in the joint of rheumatoid arthritis.
Regulatory T Cell Specificity Directs Tolerance versus Allergy against Aeroantigens in Humans.
Downregulation of RUNX3 moderates the frequency of Th17 and Th22 cells in patients with psoriasis.
A Quantitative Analysis of Complexity of Human Pathogen-Specific CD4 T Cell Responses in Healthy M. tuberculosis Infected South Africans.
Polycyclic aromatic hydrocarbons reciprocally regulate IL-22 and IL-17 cytokines in peripheral blood mononuclear cells from both healthy and asthmatic subjects.
Interleukin-25 Mediates Transcriptional Control of PD-L1 via STAT3 in Multipotent Human Mesenchymal Stromal Cells (hMSCs) to Suppress Th17 Responses.
NCR(+)ILC3 concentrate in human lung cancer and associate with intratumoral lymphoid structures.
Identification of immunogenic antigens from Aspergillus fumigatus by direct multiparameter characterization of specific conventional and regulatory CD4+ T cells.
Neutrophils in antiretroviral therapy-controlled HIV demonstrate hyperactivation associated with a specific IL-17/IL-22 environment.
CCR5 is a receptor for Staphylococcus aureus leukotoxin ED.
Early and nonreversible decrease of CD161++ /MAIT cells in HIV infection.
Increased frequencies of Th22 cells as well as Th17 cells in the peripheral blood of patients with ankylosing spondylitis and rheumatoid arthritis.
Cassotta A, Goldstein JD, Durini G, Jarrossay D, Baggi Menozzi F, Venditti M, Russo A, Falcone M, Lanzavecchia A, Gagliardi MC, Latorre D, Sallusto F
European journal of immunology 2021 Mar;51(3):648-661
European journal of immunology 2021 Mar;51(3):648-661
An immunoregulatory and tissue-residency program modulated by c-MAF in human TH17 cells.
Aschenbrenner D, Foglierini M, Jarrossay D, Hu D, Weiner HL, Kuchroo VK, Lanzavecchia A, Notarbartolo S, Sallusto F
Nature immunology 2018 Oct;19(10):1126-1136
Nature immunology 2018 Oct;19(10):1126-1136
Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.
Chen X, Wang Y, Wang J, Wen J, Jia X, Wang X, Zhang H
Oncology letters 2018 Jul;16(1):253-261
Oncology letters 2018 Jul;16(1):253-261
Th1 is the predominant helper T cell subset that produces GM-CSF in the joint of rheumatoid arthritis.
Yamada H, Haraguchi A, Sakuraba K, Okazaki K, Fukushi JI, Mizu-Uchi H, Akasaki Y, Esaki Y, Kamura S, Fujimura K, Kondo M, Miyahara H, Nakashima Y, Yoshikai Y
RMD open 2017;3(1):e000487
RMD open 2017;3(1):e000487
Regulatory T Cell Specificity Directs Tolerance versus Allergy against Aeroantigens in Humans.
Bacher P, Heinrich F, Stervbo U, Nienen M, Vahldieck M, Iwert C, Vogt K, Kollet J, Babel N, Sawitzki B, Schwarz C, Bereswill S, Heimesaat MM, Heine G, Gadermaier G, Asam C, Assenmacher M, Kniemeyer O, Brakhage AA, Ferreira F, Wallner M, Worm M, Scheffold A
Cell 2016 Nov 3;167(4):1067-1078.e16
Cell 2016 Nov 3;167(4):1067-1078.e16
Downregulation of RUNX3 moderates the frequency of Th17 and Th22 cells in patients with psoriasis.
Fu D, Song X, Hu H, Sun M, Li Z, Tian Z
Molecular medicine reports 2016 Jun;13(6):4606-12
Molecular medicine reports 2016 Jun;13(6):4606-12
A Quantitative Analysis of Complexity of Human Pathogen-Specific CD4 T Cell Responses in Healthy M. tuberculosis Infected South Africans.
Lindestam Arlehamn CS, McKinney DM, Carpenter C, Paul S, Rozot V, Makgotlho E, Gregg Y, van Rooyen M, Ernst JD, Hatherill M, Hanekom WA, Peters B, Scriba TJ, Sette A
PLoS pathogens 2016 Jul;12(7):e1005760
PLoS pathogens 2016 Jul;12(7):e1005760
Polycyclic aromatic hydrocarbons reciprocally regulate IL-22 and IL-17 cytokines in peripheral blood mononuclear cells from both healthy and asthmatic subjects.
Plé C, Fan Y, Ait Yahia S, Vorng H, Everaere L, Chenivesse C, Balsamelli J, Azzaoui I, de Nadai P, Wallaert B, Lazennec G, Tsicopoulos A
PloS one 2015;10(4):e0122372
PloS one 2015;10(4):e0122372
Interleukin-25 Mediates Transcriptional Control of PD-L1 via STAT3 in Multipotent Human Mesenchymal Stromal Cells (hMSCs) to Suppress Th17 Responses.
Wang WB, Yen ML, Liu KJ, Hsu PJ, Lin MH, Chen PM, Sudhir PR, Chen CH, Chen CH, Sytwu HK, Yen BL
Stem cell reports 2015 Sep 8;5(3):392-404
Stem cell reports 2015 Sep 8;5(3):392-404
NCR(+)ILC3 concentrate in human lung cancer and associate with intratumoral lymphoid structures.
Carrega P, Loiacono F, Di Carlo E, Scaramuccia A, Mora M, Conte R, Benelli R, Spaggiari GM, Cantoni C, Campana S, Bonaccorsi I, Morandi B, Truini M, Mingari MC, Moretta L, Ferlazzo G
Nature communications 2015 Sep 23;6:8280
Nature communications 2015 Sep 23;6:8280
Identification of immunogenic antigens from Aspergillus fumigatus by direct multiparameter characterization of specific conventional and regulatory CD4+ T cells.
Bacher P, Kniemeyer O, Teutschbein J, Thön M, Vödisch M, Wartenberg D, Scharf DH, Koester-Eiserfunke N, Schütte M, Dübel S, Assenmacher M, Brakhage AA, Scheffold A
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3332-43
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3332-43
Neutrophils in antiretroviral therapy-controlled HIV demonstrate hyperactivation associated with a specific IL-17/IL-22 environment.
Campillo-Gimenez L, Casulli S, Dudoit Y, Seang S, Carcelain G, Lambert-Niclot S, Appay V, Autran B, Tubiana R, Elbim C
The Journal of allergy and clinical immunology 2014 Nov;134(5):1142-52.e5
The Journal of allergy and clinical immunology 2014 Nov;134(5):1142-52.e5
CCR5 is a receptor for Staphylococcus aureus leukotoxin ED.
Alonzo F 3rd, Kozhaya L, Rawlings SA, Reyes-Robles T, DuMont AL, Myszka DG, Landau NR, Unutmaz D, Torres VJ
Nature 2013 Jan 3;493(7430):51-5
Nature 2013 Jan 3;493(7430):51-5
Early and nonreversible decrease of CD161++ /MAIT cells in HIV infection.
Cosgrove C, Ussher JE, Rauch A, Gärtner K, Kurioka A, Hühn MH, Adelmann K, Kang YH, Fergusson JR, Simmonds P, Goulder P, Hansen TH, Fox J, Günthard HF, Khanna N, Powrie F, Steel A, Gazzard B, Phillips RE, Frater J, Uhlig H, Klenerman P
Blood 2013 Feb 7;121(6):951-61
Blood 2013 Feb 7;121(6):951-61
Increased frequencies of Th22 cells as well as Th17 cells in the peripheral blood of patients with ankylosing spondylitis and rheumatoid arthritis.
Zhang L, Li YG, Li YH, Qi L, Liu XG, Yuan CZ, Hu NW, Ma DX, Li ZF, Yang Q, Li W, Li JM
PloS one 2012;7(4):e31000
PloS one 2012;7(4):e31000
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of Th17-polarized normal human peripheral blood cells treated with Brefeldin A (Product # 00-4506-51) (left) or restimulated with PMA/Ionomycin in the presence of Brefeldin A (right) with Anti-Human IL-17F PE (Product # 12-7169-42) and Anti-Human IL-22 PerCP-eFluor® 710. Cells in the lymphocyte gate were used for analysis.