PA5-22178

antibody from Invitrogen Antibodies

Targeting: PKLR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22178

Provider

Invitrogen Antibodies

Product name

PKLR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, A375, rat liver, PKLR-transfected 293T.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PKLR was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PKLR Polyclonal Antibody (Product # PA5-22178) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PKLR Polyclonal Antibody (Product # PA5-22178). Sample (30 µg of whole cell lysate). A: 293T. B: A431. C: HeLa. D: HepG2. E: A375. 7.5% SDS PAGE. PKLR Polyclonal Antibody (Product # PA5-22178) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PKLR Polyclonal Antibody detects Pyruvate Kinase (liver/RBC) protein by Western blot analysis. A. 20 µg Rat liver lysate/extract. 7.5% SDS-PAGE. PKLR Polyclonal Antibody (Product # PA5-22178) dilution: 1:10,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PKLR was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PKLR Polyclonal Antibody (Product # PA5-22178) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of PKLR was performed in paraffin-embedded rat liver tissue using PKLR Polyclonal Antibody (Product # PA5-22178) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Pyruvate Kinase (liver/RBC) (Product # PA5-22178) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.