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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- PA5-31313 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-Progesterone Receptor (Ser554) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-31313 targets Phospho-Progesterone Receptor pSer554 in IHC (P), IP, and WB applications and shows reactivity with Human samples. The PA5-31313 immunogen is synthetic peptide corresponding to a region within amino acids 549 and 561 of Human Progesterone Receptor.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.61 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-Progesterone Receptor pSer554 using 30µg of IMR32 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Phospho-Progesterone Receptor pSer554 polyclonal antibody (Product # PA5-31313) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Progesterone receptor (phospho Ser554) in Mouse tissue extract (50 µg). Samples was separated by 7.5% SDS-PAGE and the membrane was probed with Progesterone Receptor Polyclonal antibody (Product # PA5-31313) at a dilution of 1:10000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-Progesterone Receptor pSer554 showing staining in the nucleus of NT2D1 cells. NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min and stained using a Phospho-Progesterone Receptor pSer554 polyclonal antibody (Product # PA5-31313) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PA5-31313 immunoprecipitates Progesterone Receptor (phospho Ser554) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 µg of preimmune rabbit IgG B. Immunoprecipitation of Progesterone Receptor (phospho Ser554) protein by 2 µg of Progesterone Receptor (phospho Ser554) antibody (Product # PA5-31313) on 7.5% SDS-PAGE.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-Progesterone Receptor pSer554 in the cytosol and nucleus of paraffin-embedded human cervical carcinoma using a Phospho-Progesterone Receptor pSer554 polyclonal antibody (Product # PA5-31313) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-Progesterone Receptor pSer554 in the cytosol and nucleus of paraffin-embedded human ovarian carcinoma using a Phospho-Progesterone Receptor pSer554 polyclonal antibody (Product # PA5-31313) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-Progesterone Receptor pSer554 in the cytosol and nucleus of paraffin-embedded human colon carcinoma using a Phospho-Progesterone Receptor pSer554 polyclonal antibody (Product # PA5-31313) at a dilution of 1:500.
