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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 649301 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#649301, RRID:AB_10640871
- Product name
- Purified anti-TdT
- Antibody type
- Monoclonal
- Antigen
- TdT
- Description
- Terminal deoxynucleotidyl transferase (TdT), also known as DNA nucleotidylexotransferase (DNTT) or terminal transferase, is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. TdT is a specialized DNA polymerase expressed early in pre-B and pre-T lymphoid cell development, and in acute lymphoblastic leukemia/lymphoma cells. TdT contributes to the generation of junctional diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. The L10-2 clone has been shown to be useful for western blotting of human and mouse TdT.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store undiluted at 4°C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- A20 mouse B cell lymphoma cell extracts (Lane 1) and Jurkat cell extracts (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-mouse TdT antibody (clone L10-2). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated
