- PA5-79400 - Invitrogen Antibodies HSD17B1 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of HSD17B1 in Lane 1: rat kidney tissue lysate, Lane 2: rat liver tissue lysate, Lane 3: 293T cell lysate, Lane 4: HeLa cell lysate. Sample was incubated with HSD17B1 polyclonal antibody (Product # PA5-79400).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HSD17B1 in Lane 1: rat kidney tissue lysate, Lane 2: rat liver tissue lysate, Lane 3: 293T cell lysate, Lane 4: HeLa cell lysate. Sample was incubated with HSD17B1 polyclonal antibody (Product # PA5-79400).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HSD17B1 in, Lane 1: human T47D whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat C6 whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with HSD17B1 Polyclonal Antibody (Product # PA5-79400) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for HSD17B1 at approximately 37-38 kDa. The expected band size for HSD17B1 is at 35 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunocytochemistry analysis of HSD17B1 using anti-HSD17B1 antibody (Product # PA5-79400). HSD17B1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 1 μg/mL rabbit anti-HSD17B1 antibody(Product # PA5-79400)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry analysis of HSD17B1 on paraffin-embedded rat ovary tissue. Sample was incubated with HSD17B1 polyclonal antibody (Product# PA5-79400).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of HSD17B1 on paraffin-embedded rat liver tissue. Sample was incubated with HSD17B1 polyclonal antibody (Product# PA5-79400).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of HSD17B1 in a paraffin-embedded section of Human Placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/mL rabbit anti-HSD17B1 antibody (Product # PA5-79400) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

PA5-79400