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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- MA3-943 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calpain S1 Monoclonal Antibody (156)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA3-943 detects calpain-28 kDa subunit from bovine platelet, skeletal and heart muscle and human tissues. This antibody does not cross-react with the human 80 kDa m-calpain subunit.
- Antibody clone number
- 156
- Concentration
- Conc. Not Determined
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of Calpain in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Calpain monoclonal antibody (Product # MA3-943) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Calpain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
