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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunoprecipitation [1]
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- Product number
- MAB8231 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB8231, RRID:AB_10615167
- Product name
- NAMPT monoclonal antibody, clone AF-1E12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant NAMPT.
- Isotype
- IgG
- Antibody clone number
- AF-1E12
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Circadian control of the NAD+ salvage pathway by CLOCK-SIRT1.
Circadian clock feedback cycle through NAMPT-mediated NAD+ biosynthesis.
TNF regulates cellular NAD+ metabolism in primary macrophages.
Nakahata Y, Sahar S, Astarita G, Kaluzova M, Sassone-Corsi P
Science (New York, N.Y.) 2009 May 1;324(5927):654-7
Science (New York, N.Y.) 2009 May 1;324(5927):654-7
Circadian clock feedback cycle through NAMPT-mediated NAD+ biosynthesis.
Ramsey KM, Yoshino J, Brace CS, Abrassart D, Kobayashi Y, Marcheva B, Hong HK, Chong JL, Buhr ED, Lee C, Takahashi JS, Imai S, Bass J
Science (New York, N.Y.) 2009 May 1;324(5927):651-4
Science (New York, N.Y.) 2009 May 1;324(5927):651-4
TNF regulates cellular NAD+ metabolism in primary macrophages.
Iqbal J, Zaidi M
Biochemical and biophysical research communications 2006 Apr 21;342(4):1312-8
Biochemical and biophysical research communications 2006 Apr 21;342(4):1312-8
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- IP-WB analysis of human NAMPT in cell lysates of three cell lines using this NAMPT monoclonal antibody, clone AF-1E12 (Cat # MAB8231) for IP and NAMPT polyclonal antibody (Cat # PAB17046) for WB.Lane 1, 2 : RajiLane 3, 4 : HL-60Lane 5, 6 : K-562
- Validation comment
- Immunoprecipitation
