Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-30940 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NAMPT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Visfatin in Mouse tissue extract (50 µg). Samples was separated by 10% SDS-PAGE and the membrane was probed with Visfatin Polyclonal antibody (Product # PA5-30940) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NAMPT Polyclonal Antibody (Product # PA5-30940). Sample (30 µg of whole cell lysate). Lane A: Raji . 7.5% SDS PAGE. NAMPT Polyclonal Antibody (Product # PA5-30940) diluted at 1:3,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NAMPT Polyclonal Antibody (Product # PA5-30940). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with NAMPT Polyclonal Antibody (Product # PA5-30940) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of NAMPT was achieved by transfecting U-87 MG with NAMPT specific siRNAs (Silencer® select Product # S19725, S19724). Western Blot analysis (Fig. a) was performed using Whole cell extracts from the NAMPT knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The Blot was probed with NAMPT Polyclonal Antibody (Product # PA5-30940, 1:4000 dilution ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to NAMPT.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-NAMPT Polyclonal Antibody (Product # PA5-30940) and a 55 kDa band corresponding to NAMPT was observed across cell lines tested. Two non-specific bands at 30 kDa and 78 kDa was also observed across cell lines tested. Whole cell extracts (50 µg lysate) of U-87 MG (Lane 1) and DU 145 (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:4000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PBEF in methanol-fixed HeLa cells using a PBEF polyclonal antibody (Product # PA5-30940) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NAMPT was performed in paraffin-embedded mouse liver tissue using NAMPT Polyclonal Antibody (Product # PA5-30940) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of NAMPT was performed in paraffin-embedded rat testis tissue using NAMPT Polyclonal Antibody (Product # PA5-30940) at a dilution of 1:500.