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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC3171C-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-17/IL-17A PerCP-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-17 in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, recombinant canine Il-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
Q16552- Isotype
- IgG
- Antibody clone number
- 41802
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-17/IL-17A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA and Calcium Ionomycin were stained with Mouse Anti-Human IL-17/IL-17A PerCP-conjugated Monoclonal Antibody (Catalog # IC3171C) and Mouse Anti-Human CD3 epsilon Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F). Quadrant markers were set based on control antibody staining (Catalog # IC002C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
