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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- PA1-24450 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-MYF5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: Jurkat, mouse skeletal muscle. Predicted reactivity: Cow: 100%; Dog: 93%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 93%. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Myogenic Factor 5 in Jurkat cell lysate using a Myogenic Factor 5 polyclonal antibody (Product # PA1-24450).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Myogenic Factor 5 in mouse skeletal muscle lysate using a Myogenic Factor 5 polyclonal antibody (Product # PA1-24450) and detected using an anti-rabbit HRP secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MYF5 was performed by loading mouse skeletal and muscle tissue. Proteins were transferred to a membrane and probed with a MYF5 Polyclonal Antibody (Product # PA1-24450) at a dilution of 1:1000. Lane1: Mouse heart lysate, Lane2: Mouse skeletal muscle lysate
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MYF5 was performed by loading Jurkat cells. Proteins were transferred to a membrane and probed with a MYF5 Polyclonal Antibody (Product # PA1-24450) at a dilution of 1 µg/mL.
