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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-88263 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PML Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AVDARYQRDY EEMASRLGRL DAVLQRIRTG SALVQRMKCY ASDQEVLDMH GFLRQALCRL RQEEPQSLQA AVRTDGFDEF KVRLQDLSSC ITQGKDAAVS KKASPEAAST PRDPIDVDLD VSNTTTAQKR KCSQTQCPRK VIKMESEEGK EARLARSSPE QPRPSTSKAV SPPHLDGPPS PRSPVIGSEV FLPNSNHVAS GAGEAEERVV VISSSEDSDA ENSCMEPMET AEPQSSPAHS SPAHSSPAHS SPVQSLLRAQ GASSLPCGTY HPPAWPPHQP AEQAATPDAE PHSEPPDHQE R; Positive Samples: DU145; Cellular Location: Cytoplasm, Cytoplasmic side, Early endosome membrane, Endoplasmic reticulum membrane, Nucleus, PML body, Peripheral membrane protein, nucleolus, nucleoplasm
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.42 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using PML Polyclonal antibody (Product # PA5-88263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from normal (control) and PML knockout (KO) HeLa cells, using PML Polyclonal antibody (Product # PA5-88263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PML in extracts of various cell lines using PML Polyclonal Antibody (Product # PA5-88263) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PML was performed in HeLa cells using PML Polyclonal Antibody (Product # PA5-88263).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PML in paraffin-embedded human lung cancer using PML Polyclonal Antibody (Product # PA5-88263) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PML in paraffin-embedded human appendix using PML Polyclonal Antibody (Product # PA5-88263) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PML in paraffin-embedded human breast cancer using PML Polyclonal Antibody (Product # PA5-88263) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PML in paraffin-embedded human esophageal using PML Polyclonal Antibody (Product # PA5-88263) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PML in paraffin-embedded human placenta using PML Polyclonal Antibody (Product # PA5-88263) at a dilution of 1:100.
