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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP12217PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP12217PU-N, RRID:AB_1770619
- Product name
- anti PML / RNF71 (Sumoylation Site)
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide PRKVIKMESEEGKEA containing a predicted sumoylation site from the central region of human PML.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of anti-PML Sumoylation Site Antibody (Cat.AP12217PU-N) in Hela cell line lysates (35ug/lane). PML (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
