NBP2-24859

antibody from Novus Biologicals

Targeting: BCLAF1 BTF, KIAA0164

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-24859

Provider

Novus Biologicals

Product name

Rabbit Polyclonal BTF Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Canine, Simian

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BTF Antibody [NBP2-24859] - analysis of human BTF in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide using BTF antibody at 0.5 ug/ml.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BTF Antibody [NBP2-24859] - Total protein from human A431 and U2Os cells and mouse 3T3 and Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-BTF (NBP2-24859) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume Chemiluminescent detection reagent (NBP2-61915)

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: BTF Antibody [NBP2-24859] - analysis of human spleen probed with BTF antibody at 10 ug/ml.