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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB100-1803 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1803, RRID:AB_10003172
- Product name
- Rabbit Polyclonal 53BP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The internal region of CtIP negatively regulates DNA end resection.
Impact of Polo-like kinase 1 inhibitors on human adipose tissue-derived mesenchymal stem cells.
DNA damage response (DDR) pathway engagement in cisplatin radiosensitization of non-small cell lung cancer.
Co-treatment with BEZ235 Enhances Sensitivity of BRCA1-negative Breast Cancer Cells to Olaparib.
BRCA1 haploinsufficiency for replication stress suppression in primary cells.
Alteration/deficiency in activation-3 (Ada3) plays a critical role in maintaining genomic stability.
Discordance between phosphorylation and recruitment of 53BP1 in response to DNA double-strand breaks.
ATM-dependent phosphorylation of 53BP1 in response to genomic stress in oxic and hypoxic cells.
The role of senescence and prosurvival signaling in controlling the oncogenic activity of FGFR2 mutants associated with cancer and birth defects.
Protein phosphatase 5 regulates the function of 53BP1 after neocarzinostatin-induced DNA damage.
RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage.
ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.
Howard SM, Ceppi I, Anand R, Geiger R, Cejka P
Nucleic acids research 2020 Jun 4;48(10):5485-5498
Nucleic acids research 2020 Jun 4;48(10):5485-5498
Impact of Polo-like kinase 1 inhibitors on human adipose tissue-derived mesenchymal stem cells.
Ritter A, Friemel A, Kreis NN, Louwen F, Yuan J
Oncotarget 2016 Dec 20;7(51):84271-84285
Oncotarget 2016 Dec 20;7(51):84271-84285
DNA damage response (DDR) pathway engagement in cisplatin radiosensitization of non-small cell lung cancer.
Sears CR, Cooney SA, Chin-Sinex H, Mendonca MS, Turchi JJ
DNA repair 2016 Apr;40:35-46
DNA repair 2016 Apr;40:35-46
Co-treatment with BEZ235 Enhances Sensitivity of BRCA1-negative Breast Cancer Cells to Olaparib.
Yi YW, Park JS, Kwak SJ, Seong YS
Anticancer research 2015 Jul;35(7):3829-38
Anticancer research 2015 Jul;35(7):3829-38
BRCA1 haploinsufficiency for replication stress suppression in primary cells.
Pathania S, Bade S, Le Guillou M, Burke K, Reed R, Bowman-Colin C, Su Y, Ting DT, Polyak K, Richardson AL, Feunteun J, Garber JE, Livingston DM
Nature communications 2014 Nov 17;5:5496
Nature communications 2014 Nov 17;5:5496
Alteration/deficiency in activation-3 (Ada3) plays a critical role in maintaining genomic stability.
Mirza S, Katafiasz BJ, Kumar R, Wang J, Mohibi S, Jain S, Gurumurthy CB, Pandita TK, Dave BJ, Band H, Band V
Cell cycle (Georgetown, Tex.) 2012 Nov 15;11(22):4266-74
Cell cycle (Georgetown, Tex.) 2012 Nov 15;11(22):4266-74
Discordance between phosphorylation and recruitment of 53BP1 in response to DNA double-strand breaks.
Harding SM, Bristow RG
Cell cycle (Georgetown, Tex.) 2012 Apr 1;11(7):1432-44
Cell cycle (Georgetown, Tex.) 2012 Apr 1;11(7):1432-44
ATM-dependent phosphorylation of 53BP1 in response to genomic stress in oxic and hypoxic cells.
Harding SM, Coackley C, Bristow RG
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2011 Jun;99(3):307-12
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2011 Jun;99(3):307-12
The role of senescence and prosurvival signaling in controlling the oncogenic activity of FGFR2 mutants associated with cancer and birth defects.
Ota S, Zhou ZQ, Link JM, Hurlin PJ
Human molecular genetics 2009 Jul 15;18(14):2609-21
Human molecular genetics 2009 Jul 15;18(14):2609-21
Protein phosphatase 5 regulates the function of 53BP1 after neocarzinostatin-induced DNA damage.
Kang Y, Lee JH, Hoan NN, Sohn HM, Chang IY, You HJ
The Journal of biological chemistry 2009 Apr 10;284(15):9845-53
The Journal of biological chemistry 2009 Apr 10;284(15):9845-53
RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage.
Sakasai R, Tibbetts R
The Journal of biological chemistry 2008 May 16;283(20):13549-55
The Journal of biological chemistry 2008 May 16;283(20):13549-55
ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.
Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER 3rd, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ
Science (New York, N.Y.) 2007 May 25;316(5828):1160-6
Science (New York, N.Y.) 2007 May 25;316(5828):1160-6
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: 53BP1 [p Ser25] Antibody [NB100-1803] - Samples: Whole cell lysate (30 ug or 15 ug for WB; 1 mg for IP, 20% of IP loaded) from 293T cells that were mock treated or treated with NCS (200 ng/ml; 1h). Affinity purified rabbit anti-Phospho 53BP1 antibody at 0.04 ug/ml (A) and 0.1 ug/ml (B) for WB. 53BP1 was immunoprecipitated using affinity purified rabbit anti-53BP1 Antibody (Catalog #NB100-304). Detection: Chemiluminescence with exposure times of 3 seconds (A and B). Bands appear at ~350 kDa as the observed molecular weight and the theoretical molecular weight is 214 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: 53BP1 [p Ser25] Antibody [NB100-1803] - FFPE sections of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- Phospho 53BP1 (S25) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: 53BP1 [p Ser25] Antibody [NB100-1803] - Flow cytometric analysis of phospho 53BP1 (S25). Jurkat cells were treated with neocarzinostatin (NCS) for 1.5 hrs. 1 Million cells were fixed, permeabilized, and stained with 1.5 ug/ml antiphospho 53BP1 (S25) (Catalog #NB100-1803) in a 150 ul reaction. Isotype control (black), untreated (red), NCS treated (blue).
