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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- PA5-23120 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-TLR3 (Tyr759) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 20 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Stimulation of TLR3 triggers release of lysosomal ATP in astrocytes and epithelial cells that requires TRPML1 channels.
Beckel JM, Gómez NM, Lu W, Campagno KE, Nabet B, Albalawi F, Lim JC, Boesze-Battaglia K, Mitchell CH
Scientific reports 2018 Apr 10;8(1):5726
Scientific reports 2018 Apr 10;8(1):5726
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis ofphosphorylated TLR3 using a Phospho-TLR3/CD283 pTyr759 polyclonal antibody (Product # PA5-23120). A. Recombinant fusion proteins containing phosphorylated (lane 1) or unphosphorylated (lane 2) tyrosines at position759 (Ab used at 0.1 µg/mL). B. Primary human retinal pigment epithelial cells treated with 21-nt-Luc-siRNA for 0 (control) or 5 min which increased pTLR3 expression.
