PA5-34940

antibody from Invitrogen Antibodies

Targeting: NFATC2 NF-ATP, NFAT1, NFATp

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34940

Provider

Invitrogen Antibodies

Product name

NFATC2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: HepG2, Juarkat, Raji, BCL-1, Raw264.7.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NFATC2 Polyclonal Antibody detects NFATC2 protein by western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract. B. 30 µg Raw264.7 whole cell lysate/extract.7.5% SDS-PAGE. NFATC2 Polyclonal Antibody (Product # PA5-34940) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NFATC2 Polyclonal Antibody detects NFATC2 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract. B. 30 µg Raji whole cell lysate/extract.7.5% SDS-PAGE. NFATC2 Polyclonal Antibody (Product # PA5-34940) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NFATC2 Polyclonal Antibody detects NFAT1 protein at cytoplasm by immunofluorescent analysis. Sample: RAW264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFAT1 protein stained by NFATC2 Polyclonal Antibody (Product # PA5-34940) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NFATC2 Polyclonal Antibody detects NFAT1 protein at cytoplasm by immunofluorescent analysis. Sample: RAW264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFAT1 protein stained by NFATC2 Polyclonal Antibody (Product # PA5-34940) diluted at 1:500. Blue: Hoechst 33342 staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NFATC2 Polyclonal Antibody immunoprecipitates NFAT1 protein in IP experiments. IP samples: Raji whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of NFAT1 protein by 4 µg NFATC2 Polyclonal Antibody (Product # PA5-34940). 7.5 % SDS-PAGE. The immunoprecipitated NFAT1 protein was detected by NFATC2 Polyclonal Antibody (Product # PA5-34940) diluted at 1:500.