MA3-077

antibody from Invitrogen Antibodies

Targeting: TAF6 MGC:8964, TAF2E, TAFII70, TAFII80, TAFII85

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA3-077

Provider

Invitrogen Antibodies

Product name

TAF6 Monoclonal Antibody (25TA-2G7)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA3-077 detects TATA Binding protein (TBP) Associated factor 6 in human and mouse samples.

Antibody clone number

25TA-2G7

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TAF6 was performed by loading 20 µg of HeLa and T47D nuclear lysates, 20 µg of MCF7 and F9 whole cell lysates, and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. TAF6 was detected at ~80 kDa using a TAF6 mouse monoclonal antibody (Product # MA3-077) at a dilution of 1:1000 in blocking buffer for overnight at 4°C on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177) at a dilution of 1:1000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078).