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antibody from Invitrogen Antibodies
Targeting: HIF1AN
DKFZp762F1811, FIH1, FLJ20615, FLJ22027
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-34852 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIF1AN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HCT116, MCF-7.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HIF1AN Polyclonal Antibody (Product # PA5-34852). Sample (30 µg of whole cell lysate). Lane A: HCT116. Lane B: MCF-7. 10% SDS PAGE. HIF1AN Polyclonal Antibody (Product # PA5-34852) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HIF1AN was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF1AN Polyclonal Antibody (Product # PA5-34852) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HIF1AN Polyclonal Antibody detects HIF1AN protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF1AN protein stained by HIF1AN Polyclonal Antibody (Product # PA5-34852) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HIF1AN Polyclonal Antibody detects HIF1AN protein at cytosol on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. HIF1AN Polyclonal Antibody (Product # PA5-34852) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
