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LearnMA5-34764
antibody from Invitrogen Antibodies
Targeting: HIF1AN
DKFZp762F1811, FIH1, FLJ20615, FLJ22027
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-34764 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIF1AN Recombinant Rabbit Monoclonal Antibody (JG37-66)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: A549, LOVO, SiHa, human prostate cancer tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG37-66
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HIF1AN in A549 and Jurkat cell lysates. Samples were incubated with HIF1AN monoclonal antibody (Product # MA5-34764), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HIF1AN in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with HIF1AN monoclonal antibody (Product # MA5-34764), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HIF1AN in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with HIF1AN monoclonal antibody (Product # MA5-34764), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HIF1AN in paraffin-embedded human prostate cancer tissue. Samples were incubated with HIF1AN monoclonal antibody (Product # MA5-34764), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of HIF1AN in LOVO cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with HIF1AN monoclonal antibody (Product # MA5-34764) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
