LS-C348693

antibody from LSBio

Targeting: ENDOG

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C348693

Provider

LSBio

Product name

Anti-Endonuclease G / ENDOG Antibody LS-C348693

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Rabbit

Vial size

100µl

Storage

Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

The western blot signal is reduced in extracts from cultured cells with EndoG gene silencing driven by shRNA interference. The antibody also detects the overexpressed protein in cell cultures. The quality of our antibody has been compared to several commercially available EndoG antibodies. Other commercial antibodies only detect a band at around 35 kDa in Western Blot. EndoG has a molecular weight of 32.6 kDa (immature) and 27,7 KDa (mature, processed at the mitochondria). We detected EndoG overexpression (OvEx) in rat primary cultured cells. We also checked endogenous EndoG expression in cells transduced with control lentivirus (scrambled, scr) and lentiviral vectors inducing specific EndoG silencing by short hairpin RNA interference (RNAi). Other commercial antibodies are reported to detect endogenous EndoG as a band of 35 kDa. However, in our hands these antibodies only detected overexpressed EndoG and an endogenous unspecific band at an apparent molecular weight bigger than EndoG. On the contrary, our antibody correctly detected both repression of endogenous EndoG silencing and EndoG overexpression.