PA5-17329

antibody from Invitrogen Antibodies

Targeting: ENDOG

Antibody data

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Validation data

ENDOG protein structure - PA5-17329 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Endonuclease G in extracts from mouse and rat liver using Endonuclease G polyclonal antibody (Product # PA5-17329).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Endonuclease G in extracts from HeLa cells transfected with mouse EndoG using Endonuclease G polyclonal antibody (Product # PA5-17329).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of ENDOG was achieved by transfecting Hep G2 with ENDOG specific siRNAs (Silencer® select Product # s194395). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the ENDOG knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with ENDOG Polyclonal Antibody (Product # PA5-17329, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ENDOG.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-ENDOG Polyclonal Antibody (Product # PA5-17329) and a 32 kDa band corresponding to ENDOG was observed across cell lines and tissue extracts tested along with an uncharacterized band at ~80 kDa in tissues. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), A549 (Lane 2), HeLa (Lane 3), PC-3 (Lane 4), tissue extracts of Mouse Liver (Lane 5), Mouse Kidney (Lane 6) and Rat Liver (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).